Keywords: March 2014 YJBM Cover.jpg en Drosophila ovary imaged with a Zeiss Lightsheet microscope Ovarioles were mounted in agarose in a capillary tube preserving the three-dimensional organization of the tissue The muscle layer expresses Fasciclin 3 and Zasp both visible as green GFP-tagged proteins The actin cytoskeleton of the muscles and the egg chambers within the muscle layer are labeled with 568-Phalloidin red and nuclei are stained with DAPI blue Lightsheet microscopy is reviewed in this issue by Joseph Wolenski and Doerthe Julich Cover image courtesy of Lynn Cooley ™s lab in the Department of Genetics Yale University 2014-02-20 04 59 22 Yale Journal of Biology and Medicine Lynn Cooley cc-zero Uploaded with UploadWizard Biology journals Medicine |