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| Keywords: MirrorImagePhageDisplay.png en This image will appear in the wikipedia peptidomimetics page Figure 3 Mirror image phage display A A target L-amino acid protein L-protein for which an L-peptide inhibitor might be available is selected The D-enantiomer protein D-protein is chemically synthesized from the same sequence using D-amino acids If the target L-protein does not require a chaperone or co-factor to fold the D-protein will mirror the conformation and properties of the L-protein but the L-peptide inhibitor will most likely have little binding affinity towards it B The synthesized D-proteins are fixed to a surface and exposed to phage displaying many different L-peptides C L-peptides that do not bind well to the surface are washed away The remaining L-peptides are sequenced D The D-enantiomer peptides D-peptides of the binding L-peptides are synthesized using the same sequence and tested against the L-proteins The D-peptide will bind the L-protein but most likely not bind the D-protein mirror own Pablo gainza Phage display Uncategorized 2015 January 3 | ||||
